Elimination of cholesterol in macrophages and endothelial cells by the sterol 27-hydroxylase mechanism: comparison with high density lipoprotein-mediated reverse …
A Babiker, O Andersson, E Lund, RJ Xiu, S Deeb… - Journal of Biological …, 1997 - ASBMB
Cultured macrophages and endothelial cells have been reported to secrete 27-oxygenated
metabolites of cholesterol. This mechanism was compared with the classical high density
lipoprotein (HDL)-dependent reverse cholesterol transport. Under standard conditions,
macrophage preparations had considerably higher capacity to secrete 27-
hydroxycholesterol and 3β-hydroxy-5-cholestenoic acid than had endothelial cells and
fibroblasts. Western blotting showed that lung macrophages contained the most sterol 27 …
metabolites of cholesterol. This mechanism was compared with the classical high density
lipoprotein (HDL)-dependent reverse cholesterol transport. Under standard conditions,
macrophage preparations had considerably higher capacity to secrete 27-
hydroxycholesterol and 3β-hydroxy-5-cholestenoic acid than had endothelial cells and
fibroblasts. Western blotting showed that lung macrophages contained the most sterol 27 …
