[HTML][HTML] Sequential monitoring and stability of ex vivo–expanded autologous and nonautologous regulatory T cells following infusion in nonhuman primates
H Zhang, H Guo, L Lu, AF Zahorchak… - American Journal of …, 2015 - Elsevier
Ex vivo–expanded cynomolgus monkey CD4+ CD25+ CD127-regulatory T cells (Treg)
maintained Foxp3 demethylation status at the Treg-specific demethylation region, and
potently suppressed T cell proliferation through three rounds of expansion. When
carboxyfluorescein succinimidyl ester-or violet proliferation dye 450-labeled autologous
(auto) and nonautologous (non-auto)-expanded Treg were infused into monkeys, the
number of labeled auto-Treg in peripheral blood declined rapidly during the first week, but …
maintained Foxp3 demethylation status at the Treg-specific demethylation region, and
potently suppressed T cell proliferation through three rounds of expansion. When
carboxyfluorescein succinimidyl ester-or violet proliferation dye 450-labeled autologous
(auto) and nonautologous (non-auto)-expanded Treg were infused into monkeys, the
number of labeled auto-Treg in peripheral blood declined rapidly during the first week, but …