Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR
Journal of clinical microbiology, 2002•Am Soc Microbiol
Viral DNA stored for extended periods can be amplified by PCR. However, it is unknown
whether stored specimens give accurate quantitative results by newer real-time PCR
techniques. We therefore compared herpes simplex virus DNA levels in specimens before
and after 16 months of storage. The levels of viral DNA remained stable whether the DNA
was stored as purified DNA or unextracted DNA in a whole specimen.
whether stored specimens give accurate quantitative results by newer real-time PCR
techniques. We therefore compared herpes simplex virus DNA levels in specimens before
and after 16 months of storage. The levels of viral DNA remained stable whether the DNA
was stored as purified DNA or unextracted DNA in a whole specimen.
Abstract
Viral DNA stored for extended periods can be amplified by PCR. However, it is unknown whether stored specimens give accurate quantitative results by newer real-time PCR techniques. We therefore compared herpes simplex virus DNA levels in specimens before and after 16 months of storage. The levels of viral DNA remained stable whether the DNA was stored as purified DNA or unextracted DNA in a whole specimen.
American Society for Microbiology