Culture of human monocyte-derived macrophages
A Kelly, AM Grabiec, MA Travis - Macrophages: Methods and Protocols, 2018 - Springer
A Kelly, AM Grabiec, MA Travis
Macrophages: Methods and Protocols, 2018•SpringerThe study of human macrophages is often hampered by access to tissue and inability of this
cell type to survive in vitro following isolation. The culture of human monocyte-derived
macrophages (MDMs) represents a tool to study macrophages, with monocytes known to
give rise to tissue macrophages influenced by certain environmental cues. Here we describe
a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and
polarization toward different macrophage phenotypes.
cell type to survive in vitro following isolation. The culture of human monocyte-derived
macrophages (MDMs) represents a tool to study macrophages, with monocytes known to
give rise to tissue macrophages influenced by certain environmental cues. Here we describe
a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and
polarization toward different macrophage phenotypes.
Abstract
The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.
Springer
